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KMID : 0855520170300020053
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Korean Journal of Physical Anthropology
2017 Volume.30 No. 2 p.53 ~ p.60
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Tryptophan Negatively Regulates IgE-mediated Mast Cell Activation
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Silwal Prashanta
Shin Keun-A
Choi Seul-Gi
Namgoong Uk
Lee Chan-Yong
Heo Jun-Young
Im Kyu
Park Jong-Il
Kim Ki-Whan
Park Seung-Gil
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Abstract
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Mast cells are major immune cells in allergy to secrete allergic mediators by a degranulation process and make and secrete inflammatory lipids and cytokines in response to antigen stimulation. An amino acid tryptophan regulates immune functions. Tryptophan ameliorates inflammatory colitis in which mast cells are engaged. However, its effects on mast cells remain to be solved.
We investigated the effect of tryptophan on IgE-mediated allergic responses in the mast cells and mice. IgEmediated passive cutaneous anaphylaxis (PCA) in mice were examined. Also IgE-mediated mast cell activation responses such as degranulation of stored granules and secretion of inflammatory lipid LTB4 and cytokines (TNF-α and IL-4) were measured.
Intraperitoneal administration of tryptophan suppressed PCA in mice. Also, in the cellular level tryptophan inhibited IgE-mediated mast cell activation such as IgE-mediated degranulation and the production of LTB4. Also, it inhibited production of inflammatory cytokines TNF-α and IL-4.
In summary, tryptophan suppressed IgE-mediated allergic activation in vivo and in vitro. Tryptophan supplementation is beneficial for IgE-mediated allergy.
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KEYWORD
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Passive cutaneous anaphylaxis, Mast cell, Degranulation, IgE, Tryptophan
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